Identification of the grape basic helix–loop–helix transcription factor family and characterization of expression patterns in response to different stresses

编号 040018802

推送时间 20190527

研究领域 森林培育 

年份 2019 

类型 期刊 

语种 英语

标题 Identification of the grape basic helix–loop–helix transcription factor family and characterization of expression patterns in response to different stresses

来源期刊 Plant Growth Regulation

期 第188期

发表时间 20190318

关键词 Genome-wide;  Evolution;  bHLHtranscriptionfactor;  Phylogeneticanalysis;  Expression;  Grape; 

摘要 Basic helix–loop–helix (bHLH) proteins comprise one of the largest transcription factors (TFs) families in plants and members have been identified in model plant species, such as Arabidopsis thaliana and Oryza sativa. However, far less is known about the evolutionary history and expression patterns of bHLH proteins in woody species, such as grape (Vitis vinifera). In this study, we identified a total of 126 bHLH genes (VvbHLH) in grape genome, which were classified into 24 subfamilies based on the phylogenetic analysis included homologs from four plant species. The VvbHLH genes belonging to the same subfamilies have similar protein motifs and exon/intron structures and synteny analysis indicated that tandem and segmental duplication events have been major contributors to the expansion of the VvbHLH family. Synteny analysis between grape and A. thaliana suggested that some bHLH members shared a common ancestor. Expression analysis of 26 VvbHLH genes, representing members of the III and IV subfamilies, revealed profiles that were tissue-specific, hormone-responsive, and responsive to abiotic and biotic stresses. Taken together, the genome-wide identification and characterization of the grape bHLH TFs provide insights into their evolutionary history and represents a resource for further functional characterization in the context of crop improvement and stress tolerance.

服务人员 孙小满

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