Double DJ-1 domain containing Arabidopsis DJ-1D is a robust macromolecule deglycase

编号 040036405

推送时间 20221010

研究领域 森林培育 

年份 2022 

类型 期刊 

语种 英语

标题 Double DJ-1 domain containing Arabidopsis DJ-1D is a robust macromolecule deglycase

来源期刊 The New phytologist

期 第364期

发表时间 20220731

关键词 Arabidopsis thaliana;  DJ-1 family proteins;  DNA repair;  advanced glycation end-products;  glycation;  methylglyoxal;  reactive carbonyls;  stress responses; 

摘要 Plants, being sessile, are prone to genotoxin-induced macromolecule damage. Among the inevitable damaging agents are reactive carbonyls that induce glycation of DNA, RNA and proteins to result in the build-up of advanced glycated end-products. However, it is unclear how plants repair glycated macromolecules. DJ-1/PARK7 members are a highly conserved family of moonlighting proteins having double domains in higher plants and single domains in other phyla.
Here we show that Arabidopsis DJ-1D offers robust tolerance to endogenous and exogenous stresses through its ability to repair glycated DNA, RNA and proteins. DJ-1D also reduced the formation of reactive carbonyls through its efficient methylglyoxalase activity. Strikingly, full-length double domain-containing DJ-1D suppressed the formation of advanced glycated end-products in yeast and plants.
DJ-1D also efficiently repaired glycated nucleic acids and nucleotides in?vitro and mitochondrial DNA in?vivo under stress, indicating the existence of a new DNA repair pathway in plants.
We propose that multi-stress responding plant DJ-1 members, often present in multiple copies among plants, probably contributed to the adaptation to a variety of endogenous and exogenous stresses.

服务人员 孙小满

服务院士 尹伟伦

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