编号
040034602
推送时间
20220606
研究领域
森林培育
年份
2022
类型
期刊
语种
英语
标题
Transcription factor dynamics in plants: Insights and technologies for in vivo imaging
来源期刊 Plant Physilology
期
第346期
发表时间
20220203
关键词
Transcription factor dynamics;
imaging technique;
fluorescent labeling;
transcription factor;
TF–DNA;
摘要
Biochemical and genetic approaches have been extensively used to study transcription factor (TF) functions, but their dynamic behaviors and the complex ways in which they regulate transcription in plant cells remain unexplored, particularly behaviors such as translocation and binding to DNA. Recent developments in labeling and imaging techniques provide the necessary sensitivity and resolution to study these behaviors in living cells. In this review, we present an up-to-date portrait of the dynamics and regulation of TFs under physiologically relevant conditions and then summarize recent advances in fluorescent labeling strategies and imaging techniques. We then discuss future prospects and challenges associated with the application of these techniques to examine TFs’ intricate dance in living plants.
服务人员
孙小满
服务院士
尹伟伦
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