Effect of exogenous additives on oxidative stress and defense system of a tree: Zanthoxylum armatum DC. under in vitro conditions

编号 040023603

推送时间 20200427

研究领域 森林培育 

年份 2020 

类型 期刊 

语种 英语

标题 Effect of exogenous additives on oxidative stress and defense system of a tree: Zanthoxylum armatum DC. under in vitro conditions

来源期刊 Plant Cell, Tissue and Organ Culture (PCTOC)

期 第236期

发表时间 20200121

关键词 Antioxidant;  Ascorbic acid;  Citric acid;  Phenolic-leaching;  Poly-vinyl-pyrrolidone;  Woody; 

摘要 Toxicity related to oxidative stress has been a concern for mature, woody tree species with high phenolic content when placed under in vitro conditions, particularly at explant establishment stage. The study of biochemical factors responsible for oxidative stress (lipid peroxidation, reactive oxygen species like hydrogen peroxide) and the defense (antioxidant enzymes and non-enzymatic factors) that the plant offers, in the tree system under in vitro environment requires special consideration. This study focuses on the effect of exogenous additives [polyvinyl pyrrolidone (PVP), citric acid (CC), ascorbic acid (AA)] and initial dark treatment on oxidative stress, defense system, DNA oxidation, total chlorophyll content for the in vitro explant establishment of a medicinal tree Zanthoxylum armatum DC., for a duration of 1-month. Exogenous additives and darkness maintained a sustained level of lipid peroxidation hydrogen peroxide, oxidized DNA content and antioxidant enzymes (superoxide dismutase, catalase, and ascorbate peroxidase) in the modified treatment throughout the in vitro experimental period, in comparison to the control. Thus, establishing a relation between exogenous antioxidants and explant oxidative stress-free condition. This can further be utilized to prevent oxidative leaching of explant of mature and woody trees in vitro.

服务人员 孙小满

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