Optimization of caulogenesis in Populus nigra under lead (Pb) stress via response surface methodology (RSM) and desirability function analysis

编号 040024103

推送时间 20200601

研究领域 森林培育 

年份 2020 

类型 期刊 

语种 英语

标题 Optimization of caulogenesis in Populus nigra under lead (Pb) stress via response surface methodology (RSM) and desirability function analysis

来源期刊 Plant Cell, Tissue and Organ Culture (PCTOC)

期 第241期

发表时间 20200418

关键词 Caulogenesis;  Lead(Pb);  Modeling;  Optimization;  Phytohormones;  Populus nigra; 

摘要 Phytoremediation is a natural soil depollution approach whereby plant species are used to absorb and accumulate toxic metals such as lead. However, phytoremediation can be affected by plant sensitivity to high concentrations of heavy metals that reduce plant phytoremediation efficiency. To overcome this drawback, and to produce tolerant plants, the somaclonal variation technique is a tool of choice to introduce genetic variations in plant cells cultured in vitro under optimized caulogenesis protocols. Using combinatory concentrations of three phytohormones (benzylaminopurine “BAP”, gibberellic acid “GA3”, and 2,4-dichlorophenoxyacetic acid “2.4-D”), we applied a caulogenesis protocol in Populus nigra under lead nitrate [Pb(NO3)2] stress conditions and selected from the resulting somaclones those that tolerate an optimal combinatory concentration of the three phytohormones. By applying small composite design (SCD), response surface method (RSM) and desirability function (D), we found that the combinatory concentrations of BAP at 6.66 μM; GA3 at 0.38 μM; and 2,4-D at 11.36 μM were optimal for poplar caulogenesis on a culture medium containing 274.40 mg/L of Pb(NO3)2. Although Pb(NO3)2 inhibited caulogenesis, 2,4-D attenuated the inhibitory effect of lead on callus recovery. BAP, in turn, attenuated the inhibitory effects on bud induction and bud number of these plants. These results suggest that poplar caulogenesis can be optimized mathematically under heavy metal stress conditions in view of breeding stress tolerant somaclones.

服务人员 孙小满

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