Structural insights into Arabidopsis ethylene response factor 96 with an extended N-terminal binding to GCC box

编号 040025501

推送时间 20200907

研究领域 森林培育 

年份 2020 

类型 期刊 

语种 英语

标题 Structural insights into Arabidopsis ethylene response factor 96 with an extended N-terminal binding to GCC box

来源期刊 Plant Molecular Biology

期 第255期

发表时间 20200819

关键词 AP2/ERF;  ERF 96;  GCC box;  EDLL motif;  Protein structure;  Fluorescence polarization assay; 

摘要 The phytohormone ethylene is widely involved in many developmental processes and is a crucial regulator of defense responses against biotic and abiotic stresses in plants. Ethylene-responsive element binding protein, a member of the APETALA2/ethylene response factor (AP2/ERF) superfamily, is a transcription factor that regulates stress-responsive genes by recognizing a specific cis-acting element of target DNA. A previous study showed only the NMR structure of the AP2/ERF domain of AtERF100 in complex with a GCC box DNA motif. In this report, we determined the crystal structure of AtERF96 in complex with a GCC box at atomic resolution. We analyzed the binding residues of the conserved AP2/ERF domain in the DNA recognition sequence. In addition to the AP2/ERF domain, an N-terminal α-helix of AtERF96 participates in DNA interaction in the flanking region. We also demonstrated the structure of AtERF96 EDLL motif, a unique conserved motif in the group IX of AP2/ERF family, might involve in the transactivation of defense-related genes. Our study establishes the structural basis of the AtERF96 transcription factor in complex with the GCC box, as well as the DNA binding mechanisms of the N-terminal α-helix and AP2/ERF domain.

服务人员 孙小满

PDF文件 浏览全文