Transcription factors ABF4 and ABR1 synergistically regulate amylase-mediated starch catabolism in drought tolerance

编号 040038103

推送时间 20230206

研究领域 森林培育 

年份 2022 

类型 期刊 

语种 英语

标题 Transcription factors ABF4 and ABR1 synergistically regulate amylase-mediated starch catabolism in drought tolerance

来源期刊 Plant Physiology

期 第381期

发表时间 20220914

关键词 ABA repressor 1;  ABRE-binding factor 4;  Trifoliata orange;  drought stress;  starchdegradation;  transcription regulation;  β-amylase 3; 

摘要 β-Amylase (BAM)-mediated starch degradation is a main source of soluble sugars that help plants adapt to environmental stresses. Here, we demonstrate that dehydration-induced expression of PtrBAM3 in trifoliate orange (Poncirus trifoliata (L.) Raf.) functions positively in drought tolerance via modulation of starch catabolism. Two transcription factors, PtrABF4 (P. trifoliata abscisic acid-responsive element-binding factor 4) and PtrABR1 (P. trifoliata ABA repressor 1), were identified as upstream transcriptional activators of PtrBAM3 through yeast one-hybrid library screening and protein–DNA interaction assays. Both PtrABF4 and PtrABR1 played a positive role in plant drought tolerance by modulating soluble sugar accumulation derived from BAM3-mediated starch decomposition. In addition, PtrABF4 could directly regulate PtrABR1 expression by binding to its promoter, leading to a regulatory cascade to reinforce the activation of PtrBAM3. Moreover, PtrABF4 physically interacted with PtrABR1 to form a protein complex that further promoted the transcriptional regulation of PtrBAM3. Taken together, our finding reveals that a transcriptional cascade composed of ABF4 and ABR1 works synergistically to upregulate BAM3 expression and starch catabolism in response to drought condition. The results shed light on the understanding of the regulatory molecular mechanisms underlying BAM-mediated soluble sugar accumulation for rendering drought tolerance in plants.

服务人员 孙小满

服务院士 尹伟伦

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