Functional characteristics of a calcium-dependent protein kinase (MaCDPK1) enduring stress tolerance from Morus atropurpurea Roxb.

编号 040023601

推送时间 20200427

研究领域 森林培育 

年份 2020 

类型 期刊 

语种 英语

标题 Functional characteristics of a calcium-dependent protein kinase (MaCDPK1) enduring stress tolerance from Morus atropurpurea Roxb.

来源期刊 Plant Cell, Tissue and Organ Culture (PCTOC)

期 第236期

发表时间 20200121

关键词 Calcium-dependent protein kinases;  Mulberry;  Salt stress;  Drought stress;  ABA; 

摘要 Calcium-dependent protein kinases (CDPKs), as an important calcium sensor in plants, are widely involved in the signal transmission process of growth and development, pathogen defense response and biotic or abiotic stress. In this study, we cloned in mulberry leaves, the full-length CDS sequence of MaCDPK1, which encodes 573 amino acids with an isoelectric point of 5.36. Bioinformatics analysis found MaCDPK1 have a classical kinase domain and an EF-hands domain. We obtained the wild-type and the mutant-type of MaCDPK1(CDPK1 and mCDPK1) by prokaryotic expression and purification. Through kinase assay, it was found that CDPK1 has intact enzymatic activity and exhibits Mg2+ and Ca2+ dependence with a Km of 30 μM and a Vmax of 100,000 [RLU]/min/μg. However, the mCDPK1 has no activity, which means that aspartic acid at position 234th is critical for the activity. The 1500 bp upstream of the start codon of the MaCDPK1 gene contains core promoter elements TAAT-box and CAAT-box, and contains response elements associated with adverse signals such as light, drought, and abscisic acid. The tissues expression level of MaCDPK1 gene in different tissues of mulberry trees under different stress conditions reflect complex expression patterns, indicating that the MaCDPK1 gene responded to stress tolerance. These results are likely to indicate that MaCDPK1 positively regulates salt and drought stress in Morus atropurpurea Roxb.

服务人员 孙小满

PDF文件 浏览全文