Multiparameter in vivo imaging in plants using genetically encoded fluorescent indicator multiplexing

编号 040032003

推送时间 20211206

研究领域 森林培育 

年份 2021 

类型 期刊 

语种 英语

标题 Multiparameter in vivo imaging in plants using genetically encoded fluorescent indicator multiplexing

来源期刊 Plant Physiology

期 第320期

发表时间 20210826

关键词 plant physiology;  biosensor analyses;  fluorescent protein;  genetically
encoded fluorescent indicators (GEFIs); 

摘要 Biological processes are highly dynamic, and during plant growth, development, and environmental interactions, they occur and influence each other on diverse spatiotemporal scales. Understanding plant physiology on an organismic scale requires analyzing biological processes from various perspectives, down to the cellular and molecular levels. Ideally, such analyses should be conducted on intact and living plant tissues. Fluorescent protein (FP)-based in vivo biosensing using genetically encoded fluorescent indicators (GEFIs) is a state-of-the-art methodology for directly monitoring cellular ion, redox, sugar, hormone, ATP and phosphatidic acid dynamics, and protein kinase activities in plants. The steadily growing number of diverse but technically compatible genetically encoded biosensors, the development of dual-reporting indicators, and recent achievements in plate-reader-based analyses now allow for GEFI multiplexing: the simultaneous recording of multiple GEFIs in a single experiment. This in turn enables in vivo multiparameter analyses: the simultaneous recording of various biological processes in living organisms. Here, we provide an update on currently established direct FP-based biosensors in plants, discuss their functional principles, and highlight important biological findings accomplished by employing various approaches of GEFI-based multiplexing. We also discuss challenges and provide advice for FP-based biosensor analyses in plants.

服务人员 孙小满

服务院士 尹伟伦

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