PI4Kγ2 interacts with E3 ligase MIEL1 to regulate auxin metabolism and root development

编号 040025705

推送时间 20200921

研究领域 森林培育 

年份 2020 

类型 期刊 

语种 英语

标题 PI4Kγ2 interacts with E3 ligase MIEL1 to regulate auxin metabolism and root development

来源期刊 Plant Physiology

期 第257期

发表时间 20200811

关键词 phosphatidylinositol 4-kinase γ2 (PI4Kγ2);  MIEL1;  MYB30;  auxin;  GH3; 

摘要 Root development is important for normal plant growth and nutrient absorption. Studies have revealed the involvement of various factors in this complex process, improving our understanding of the relevant regulatory mechanisms. Here, we functionally characterize the role of Arabidopsis phosphatidylinositol 4-kinase γ2 (PI4Kγ2) in root elongation regulation, which functions to modulate stability of the RING-type E3 ligase MYB30-INTERACTING E3 LIGASE 1 (MIEL1) and auxin metabolism. Mutant plants deficient in PI4Kγ2 (pi4kγ2) exhibited a shortened root length and elongation zone due to reduced auxin level. PI4Kγ2 was shown to interact with MIEL1, regulating its degradation and further the stability of transcription factor MYB30, which suppresses auxin metabolism by directly binding to promoter regions of GH3.2 and GH3.6. Interestingly, pi4kγ2 plants presented altered hypersensitive response, indicating that PI4Kγ2 regulates synergetic growth and defense of plants through modulating auxin metabolism. These results reveal the importance of protein interaction in regulating ubiquitin-mediated protein degradation in eukaryotic cells, and illustrate a mechanism coordinating plant growth and biotic stress response.

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