H2Bub1 regulates RbohD-dependent H2O2 signal pathway in the defense responses to Verticillium dahliae toxins

编号 040021905

推送时间 20191230

研究领域 森林培育 

年份 2019 

类型 期刊 

语种 英语

标题 H2Bub1 regulates RbohD-dependent H2O2 signal pathway in the defense responses to Verticillium dahliae toxins

来源期刊 Plant Physiology

期 第219期

发表时间 20191030

关键词 H2Bub1;  Verticillium dahliae toxins;  NADPH oxidase; 

摘要 Histone H2B monoubiquitination (H2Bub1) plays critical roles in regulating growth and development as well as stress responses in Arabidopsis (Arabidopsis thaliana). In this study, we used wild-type and HUB1 and HUB2 loss-of-function Arabidopsis plants to elucidate the mechanisms involved in the regulation of the plant's defense responses to Verticillium dahliae toxins (Vd-toxins). We demonstrated that HUB-mediated H2Bub1 regulates the expression of NADPH oxidase RbohD by enhancing the enrichment of histone H3 tri-methylated on K4 in response to Vd-toxins. RbohD-dependent hydrogen peroxide (H2O2) signaling is a critical modulator in the defense response against Vd-toxins. Moreover, H2Bub1 also affects posttranscriptional mitogen-activated protein kinase (MAPK, or MPK) signaling. H2Bub1 was required for the activation of MPK3 and MPK6. MPK3 and MPK6 are involved in regulating RbohD-mediated H2O2 production. MPK3 and MPK6 are associated with protein Tyr phosphatases (PTPs), such as Tyr-specific phosphatase 1 and mitogen-activated protein kinases phosphatase 1, which negatively regulated H2O2 production. In addition, H2Bub1 is involved in regulating the expression of WRKY33. WRKY33 directly binds to RbohD and functions as a transcription factor to regulate the expression of RbohD. Collectively, our results indicate that H2Bub1 regulates NADPH oxidase RbohD-dependent H2O2 production, and the PTPs-MPK3/6-WRKY pathway plays an important role in the regulation of RbohD-dependent H2O2 signaling in defense responses to Vd-toxins in Arabidopsis.

服务人员 孙小满

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