Pathogen-associated Molecular Pattern-triggered Immunity Involves Proteolytic Degradation of Core Nonsense-mediated mRNA Decay Factors During the Early Defense Response

编号 040023204

推送时间 20200330

研究领域 森林培育 

年份 2020 

类型 期刊 

语种 英语

标题 Pathogen-associated Molecular Pattern-triggered Immunity Involves Proteolytic Degradation of Core Nonsense-mediated mRNA Decay Factors During the Early Defense Response

来源期刊 The Plant Cell

期 第232期

发表时间 20200221

关键词 UPF protein ubiquitination;  resistance gene;  Nonsense-mediated mRNA decay (NMD);  Defense response;  Effective immunity; 

摘要 Nonsense-mediated mRNA decay (NMD), an mRNA quality control process, has been implicated in plant immunity. A subset of fully spliced (FS) transcripts of Arabidopsis resistance (R) genes are reportedly upregulated during bacterial infection. Here, we report that 81.2% and 65.1% of FS natural TIR-NBS-LRR (TNL) and CC-NBS-LRR (CNL) transcripts, respectively, retained characteristics of NMD regulation, by which their transcript levels could be controlled posttranscriptionally. Both bacterial infection and perception of bacteria by pattern recognition receptors (PRRs) initiated the destruction of UPF1, UPF2, and UPF3 within 30 minutes of inoculation via independent ubiquitination of UPF1 and UPF3 and the 26S proteasome pathway. Induction of UPF1 and UPF3 ubiquitination was delayed specifically in mpk3 or mpk6, but not in SA-signaling mutants, during the early immune response. Finally, previously uncharacterized TNL-type R transcripts accumulated in upf mutants conferred disease resistance to infection with a virulent Pseudomonas strain in plants. Our findings demonstrate that NMD is one of the main regulatory processes through which PRRs can fine-tune R transcript levels to reduce fitness costs and achieve effective immunity.

服务人员 孙小满

PDF文件 浏览全文