SMC5/6 Complex Subunit NSE4A is Involved in DNA Damage Repair and Seed Development in Arabidopsis

编号 040019502

推送时间 20190715

研究领域 森林培育 

年份 2019 

类型 期刊 

语种 英语

标题 SMC5/6 Complex Subunit NSE4A is Involved in DNA Damage Repair and Seed Development in Arabidopsis

来源期刊 Plant Cell

期 第195期

发表时间 20190429

关键词 Seed Development;  DNA Damage;  NSE4A subunit;  SMC5/6 Complex; 

摘要 The maintenance of genome integrity over cell divisions is critical for plant development and the correct transmission of genetic information to the progeny. A key factor involved in this process is the STRUCTURAL MAINTAINANCE OF CHROMOSOME 5/6 (SMC5/6) complex, related to cohesin and condensin complexes that control sister chromatid alignment and chromosome condensation, respectively. Here we characterize NON-SMC ELEMENT4 (NSE4) paralogs of the SMC5/6 complex in Arabidopsis. NSE4A is expressed in meristems and accumulates during DNA damage repair. Partial loss-of-function nse4a mutants are viable but hypersensitive to DNA damage induced by zebularine. In addition, nse4a mutants produce abnormal seeds, with non-cellularized endosperm and embryos that maximally develop to the heart or torpedo stage. This resembles defects in cohesin and condensin mutants and suggests a role for all three SMC complexes in differentiation during seed development. In contrast, NSE4B is expressed only in a few cell types and loss-of-function mutants do not have any obvious phenotype. In summary, our study shows that the NSE4A subunit of the SMC5/6 complex is essential for DNA damage repair in somatic tissues, and plays a role in plant reproduction.

服务人员 孙小满

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