RNA interference-independent reprogramming of DNA methylation in Arabidopsis

编号 040027005

推送时间 20201221

研究领域 森林培育 

年份 2020 

类型 期刊 

语种 英语

标题 RNA interference-independent reprogramming of DNA methylation in Arabidopsis

来源期刊 Nature Plants

期 第270期

发表时间 20201130

关键词 Arabidopsis;  RNA interference;  DNA methylation; 

摘要 DNA methylation is important for silencing transposable elements (TEs) in diverse eukaryotes, including plants. In plant genomes, TEs are silenced by methylation of histone H3 lysine 9 (H3K9) and cytosines in both CG and non-CG contexts. The role of RNA interference (RNAi) in establishing TE-specific silent marks has been extensively studied, but the importance of RNAi-independent pathways remains largely unexplored. Here, we directly investigated transgenerational de novo DNA methylation of TEs after the loss of silent marks. Our analyses uncovered potent and precise RNAi-independent pathways for recovering non-CG methylation and H3K9 methylation in most TE genes (that is, coding regions within TEs). Characterization of a subset of TE genes without the recovery revealed the effects of H3K9 demethylation, replacement of histone H2A variants and their interaction with CG methylation, together with feedback from transcription. These chromatin components are conserved among eukaryotes and may contribute to chromatin reprogramming in a conserved manner.

服务人员 孙小满

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