Future prospects for investigating ciliate biodiversity

编号 030038001

推送时间 20230130

研究领域 森林经理 

年份 2022 

类型 期刊 

语种 英语

标题 Future prospects for investigating ciliate biodiversity

来源期刊 ACTA PROTOZOOLOGICA

期 第380期

发表时间 20221228

关键词 Biodiversity;  Ciliophora;  fluorescence;  staining methods; 

摘要 Ciliates have a long history of being central in evolutionary and ecological studies on eukaryotic microorganisms. Although thousands of species have been discovered, their total diversity still remains unknown. Here, we will discuss two unsolved problems that hinder the further exploration of ciliate diversity at the species level, and potential solutions to these problems are proposed. First, ciliate morphospecies are difficult to identify because the different silver stains are not scalable (they do not represent high-throughput methods) and basic supplies are lacking (e.g., protargol); a solution may be the development of fluorescent staining techniques. Second, ciliate phylogenetic species are difficult to identify because of extensive paralogy in nuclear-protein-coding genes; a solution may be to concentrate on sequencing mitochondrial genomes. These two approaches could be integrated into a high-throughput fluorescent-single-cell sorting and mitochondrial genomes sequencing process that would enable the observation and better understanding of ciliate species on a massive scale.

服务人员 付贺龙

服务院士 唐守正

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