TRANSTHYRETIN-LIKE and BYPASS1-LIKE co-regulate growth and cold tolerance in Arabidopsis

编号 040025502

推送时间 20200907

研究领域 森林培育 

年份 2020 

类型 期刊 

语种 英语

标题 TRANSTHYRETIN-LIKE and BYPASS1-LIKE co-regulate growth and cold tolerance in Arabidopsis

来源期刊 BMC Plant Biology

期 第255期

发表时间 20200714

关键词 BYPASS1-LIKE;  TRANSTHYRETIN-LIKE;  Plant growth;  Cold tolerance;  Arabidopsis; 

摘要 Background: Cold stress inhibits normal physiological metabolism in plants, thereby seriously affecting plant
development. Meanwhile, plants also actively adjust their metabolism and development to adapt to changing
environments. Several cold tolerance regulators have been found to participate in the regulation of plant
development. Previously, we reported that BYPASS1-LIKE (B1L), a DUF793 family protein, participates in the regulation
of cold tolerance, at least partly through stabilizing C-REPEAT BINDING FACTORS (CBFs). In this study, we found that
B1L interacts with TRANSTHYRETIN-LIKE (TTL) protein, which is involved in brassinosteroid (BR)-mediated plant growth
and catalyses the synthesis of S-allantoin, and both proteins participate in modulating plant growth and cold tolerance.
Results: The results obtained with yeast two hybrid (Y2H) and bimolecular fluorescence complementation (BiFC)
assays showed that B1L directly interacted with TTL. Similar to the ttl-1 and ttl-2 mutants, the b1l mutant
displayed a longer hypocotyl and greater fresh weight than wild type, whereas B1L-overexpressing lines exhibited
a shorter hypocotyl and reduced fresh weight. Moreover, ttl-1 displayed freezing tolerance to cold treatment
compared with WT, whereas the b1l mutant and TTL-overexpressing lines were freezing-sensitive. The b1l ttl
double mutant had a developmental phenotype and freezing tolerance that were highly similar to those of ttl-1
compared to b1l, indicating that TTL is important for B1L function. Although low concentrations of brassinolide
(0.1 or 1 nM) displayed similarly promoted hypocotyl elongation of WT and b1l under normal temperature, it
showed less effect to the hypocotyl elongation of b1l than to that of WT under cold conditions. In addition, the
b1l mutant also contained less amount of allantoin than Col-0.
Conclusion: Our results indicate that B1L and TTL co-regulate development and cold tolerance in Arabidopsis,
and BR and allantoin may participate in these processes through B1L and TTL.

服务人员 孙小满

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