RBP differentiation contributes to selective transmissibility of OPT3 mRNAs

编号 040032302

推送时间 20211227

研究领域 森林培育 

年份 2021 

类型 期刊 

语种 英语

标题 RBP differentiation contributes to selective transmissibility of OPT3 mRNAs

来源期刊 Plant physiology

期 第323期

发表时间 20210802

关键词 plant development;  stress tolerance;  OPT3;  mRNA;  mRNA mobility;  RBP; 

摘要 Long-distance mobile mRNAs play key roles in gene regulatory networks that control plant development and stress tolerance. However, the mechanisms underlying species-specific delivery of mRNA still need to be elucidated. Here, the use of grafts involving highly heterozygous apple (Malus) genotypes allowed us to demonstrate that apple (Malus domestica) oligopeptide transporter3 (MdOPT3) mRNA can be transported over a long distance, from the leaf to the root, to regulate iron uptake; however, the mRNA of Arabidopsis (Arabidopsis thaliana) oligopeptide transporter 3 (AtOPT3), the MdOPT3 homolog from A. thaliana, does not move from shoot to root. Reciprocal heterologous expression of the two types of mRNAs showed that the immobile AtOPT3 became mobile and moved from the shoot to the root in two woody species, Malus and Populus, while the mobile MdOPT3 became immobile in two herbaceous species, A. thaliana and tomato (Solanum lycopersicum). Furthermore, we demonstrated that the different transmissibility of OPT3 in A. thaliana and Malus might be caused by divergence in RNA-binding proteins between herbaceous and woody plants. This study provides insights into mechanisms underlying differences in mRNA mobility and validates the important physiological functions associated with this process.

服务人员 孙小满

服务院士 尹伟伦

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