Osmotic adjustments support growth of poplar cultured cells under high concentrations of carbohydrates

编号 040024601

推送时间 20200706

研究领域 森林培育 

年份 2020 

类型 期刊 

语种 英语

标题 Osmotic adjustments support growth of poplar cultured cells under high concentrations of carbohydrates

来源期刊 Plant Cell Reports

期 第246期

发表时间 20200420

关键词 Cryo-SEM;  In vitro culture;  Mineral elements;  Non-structural carbohydrates;  Osmotic balance; 

摘要 Osmotic stress is caused by several abiotic factors such as drought, salinity, or freezing. However, the threshold of osmotic potential that allows the growth under stress conditions has not been thoroughly studied. In this study, different levels of osmotic stress in Populus alba (L.) callus have been induced with the addition of mannitol or sorbitol in the medium (from 0 to 500 mM). The key factor for preserving the growth was observed to be the restoration of a constant difference in osmotic potential between callus and medium for all the tested conditions. The osmotic adjustments were primarily achieved with the uptake of mannitol or sorbitol from the media considering their chemical properties instead of their biological functions. The decrease in water content (from ? 1 to ? 10% after 21 days) and mineral elements, such as potassium, calcium, and magnesium, together with the alterations in cell morphology, did not show negative effects on growth. The activity of sorbitol dehydrogenase was detected for the first time in poplar (+?4.7 U l?1 in callus treated with sorbitol compared to control callus). This finding suggested the importance of choosing carefully the molecules used to exert osmotic stress for separating the dual function of carbohydrates in osmotic adjustments and cell metabolism.

服务人员 孙小满

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