Separation of Panax notoginseng saponins on modified rosin ester-bonded silica stationary phase and its mechanism

编号 020038001

推送时间 20230130

研究领域 林产化工 

年份 2022 

类型 期刊 

语种 英语

标题 Separation of Panax notoginseng saponins on modified rosin ester-bonded silica stationary phase and its mechanism

来源期刊 Analytica Chimica Acta

期 第380期

发表时间 20230125

关键词 Modified rosin ester-bonded silica;  Panax notoginseng saponins;  Stationary phase;  Separation; 

摘要 A column prepared using a unique three-membered phenanthrene skeleton of rosin has complementary selec-tivity to a C18 column for some separation tasks. In this study, propylene pimaric acid (16-hydroxyethyl acrylate-34-n-butyl) ester (BRB) and propylene pimaric acid (16-hydroxyethyl acrylate-34-dodecyl) ester (BRLA) were used as functional ligands to prepare two novel stationary phases, namely BRB@SiO2 and BRLA@SiO2, through a "thiol-ene" click chemistry reaction. The characterization results of Fourier transform infrared spectroscopy, thermogravimetric analysis, nitrogen adsorption-desorption measurements, and contact angle tests showed that the BRB@SiO2 and BRLA@SiO2 stationary phases were successfully prepared. In addition, the performance of the columns was evaluated using the Tanaka test and hydrophobic subtraction model, which showed that the sta-tionary phases exhibited typical reversed-phase chromatography performance and good hydrophobicity, hy-drophobic selectivity, and steric selectivity. The changes in the retention of Panax notoginseng saponins on a column under different chromatographic conditions (acetonitrile content, flow rate, and column temperature) were investigated. The separation effect of BRB@SiO2 and BRLA@SiO2 columns on P. notoginseng saponins was better than that of the C18 column and the BRLA@SiO2 column could replace the C18 column for the detection of P. notoginseng saponins.

服务人员 尚玮姣

服务院士 宋湛谦

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