编号 040021101
推送时间 20191104
研究领域 森林培育
年份 2019
类型 期刊
语种 英语
标题 Universal stress protein in Malus sieversii confers enhanced drought tolerance
来源期刊 Journal of Plant Research
期 第211期
发表时间 20190903
关键词 M.sieversii; Transcriptome; MsUspA; Drought str;
摘要 Drought is an important environmental factor that can severely affect plant growth and reproduction. Although many genes related to drought tolerance have been studied in economically important crops, very few genes have been functionally identified in Malus sieversii. In this study, we isolated a new gene based on throughput RNA sequencing analysis and constructed genetic expression vectors and transformed in Arabidopsis thaliana for functional verification. The results showed that MsUspA ectopic expression driven by constitutive (CaMV 35S) promoter gave rise to substantial improvements in ability of transgenic A. thaliana plants to survive under extreme drought conditions. Improved drought resistance mainly depends on more compact cellular structure, longer roots, strong resilience and low-level ROS. Molecular expression analysis showed that MsUspA may be involved in hormone and secondary metabolite synthesis regulation to improve drought resistance.
服务人员 孙小满
PDF文件 浏览全文