The stress induced caleosin, RD20/CLO3, acts as a negative regulator of GPA1 in Arabidopsis

编号 040032104

推送时间 20211213

研究领域 森林培育 

年份 2021 

类型 期刊 

语种 英语

标题 The stress induced caleosin, RD20/CLO3, acts as a negative regulator of GPA1 in Arabidopsis

来源期刊 Plant Molecular Biology

期 第321期

发表时间 20211002

关键词 Heterotrimeric G-protein alpha subunit;  Gα protein;  GPA1;  Calcium-binding protein;  RD20/CLO3;  Signal
transduction;  Protein–protein interaction;  Etiolation; 

摘要 Heterotrimeric G proteins and calcium signaling have both been shown to play a role in the response to environmental abiotic stress in plants; however, the interaction between calcium-binding proteins and G-protein signaling molecules remains elusive. We investigated the interaction between the alpha subunit of the heterotrimeric G-protein complex, GPA1, of Arabidopsis thaliana with the calcium-binding protein, the caleosin RD20/CLO3, a gene strongly induced by drought, salt and abscisic acid. The proteins were found to interact in vivo by bimolecular fluorescent complementation (BiFC); the interaction was localized to the endoplasmic reticulum and to oil bodies within the cell. The constitutively GTP-bound GPA1 (GPA1QL) also interacts with RD20/CLO3 as well as its EF-hand mutant variations and these interactions are localized to the plasma membrane. The N-terminal portion of RD20/CLO3 was found to be responsible for the interaction with GPA1 and GPA1QL using both BiFC and yeast two-hybrid assays. RD20/CLO3 contains a single calcium-binding EF-hand in the N-terminal portion of the protein; disruption of the calcium-binding capacity of the protein obliterates interaction with GPA1 in in vivo assays and decreases the interaction between the caleosin and the constitutively active GPA1QL. Analysis of rd20/clo3 mutants shows that RD20/CLO3 plays a key role in the signaling pathway controlling hypocotyl length in dark grown seedlings and in leaf morphology. Our findings indicate a novel role for RD20/CLO3 as a negative regulator of GPA1.

服务人员 孙小满

服务院士 尹伟伦

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