Integrative analysis from the epigenome to translatome uncovers patterns of dominant nuclear regulation during transient stress

编号 040020904

推送时间 20191021

研究领域 森林培育 

年份 2019 

类型 期刊 

语种 英语

标题 Integrative analysis from the epigenome to translatome uncovers patterns of dominant nuclear regulation during transient stress

来源期刊 The Plant Cell

期 第209期

发表时间 20190913

关键词 epigenome;  dominant nuclear regulation;  transient stress;  post-transcriptional gene regulation;  hypoxia reveals; 

摘要 Gene regulation is modulated from chromatin to translation. To better understand the integration of nuclear and cytoplasmic gene regulatory dynamics, we performed a multi-omic survey of the epigenome through the translatome of the response of Arabidopsis seedlings to hypoxia and reoxygenation. This included eight assays of chromatin (histones, accessibility, RNAPII and transcription factor binding) and three assays of RNA (nuclear, polyadenylated, and ribosome-associated). Dynamic patterns of nuclear regulation distinguished stress-induced and growth-associated mRNAs. Rapid upregulation of hypoxia-responsive gene transcripts and their preferential translation was generally accompanied by increased chromatin accessibility, RNAPII engagement and reduced Histone 2A.Z association. Hypoxia promoted a progressive upregulation of heat stress gene transcripts, evident from engagement of RNAPII and an increase in nuclear RNA, with polyadenylated RNAs only elevated following prolonged hypoxic stress or reoxygenation. Promoters of the rapidly versus progressively upregulated gene cohorts were enriched for cis-elements of ethylene-responsive and heat shock factor transcription factor families, respectively. By contrast, genes associated with growth including the majority that encode cytosolic ribosomal proteins underwent distinct histone modifications, yet retained RNAPII engagement and accumulated nuclear transcripts during the stress. Upon reaeration, many of the progressively upregulated and growth-associated gene transcripts were rapidly mobilized to ribosomes. Thus, multi-level nuclear regulation distinguishes transcript synthesis, accumulation and translation in response to a transient stress.

服务人员 孙小满

PDF文件 浏览全文