Identification and functional characterization of MdPIF3 in response to cold and drought stress in Malus domestica

编号 040027404

推送时间 20210118

研究领域 森林培育 

年份 2021 

类型 期刊 

语种 英语

标题 Identification and functional characterization of MdPIF3 in response to cold and drought stress in Malus domestica

来源期刊 Plant Cell, Tissue and Organ Culture (PCTOC)

期 第274期

发表时间 20201103

关键词 mGWAS;  Omics;  Systems biology;  Extreme temperature;  Zero hunger; 

摘要 PHYTOCHROME INTERACTING FACTORs (PIFs) are a subset of helix-loop-helix (bHLH) transcription factors, which play critical roles in plant growth and development, as well as in adaption to ambient environments. However, PIF members have not been completely identified in apple (Malus domestica), a widely distributed fruit crop with significant economical importance. Here, we characterized MdPIF3, the homolog of AtPIF3, and determined its role in response to abiotic stresses in apple. We first analyzed its gene and protein structure, and found that it contained bHLH domain, active phytochrome B binding (APB) motif, as well as active phytochrome A binding (APA) motif. Yeast-two-hybrid assays indicated that MdPIF3 formed a homodimer by itself and heterodimers with other MdPIFs. Moreover, MdPIF3 was responsive to light and cold treatment at both transcriptional and post-translational levels. Overexpression of MdPIF3 reduced cold tolerance but enhanced drought resistance in both apple callus and Arabidopsis

服务人员 孙小满

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