Genome-wide search and structural and functional analyses for late embryogenesis-abundant (LEA) gene family in poplar

编号 040028404

推送时间 20210329

研究领域 森林培育 

年份 2021 

类型 期刊 

语种 英语

标题 Genome-wide search and structural and functional analyses for late embryogenesis-abundant (LEA) gene family in poplar

来源期刊 BMC Plant Biology

期 第284期

发表时间 20210224

关键词 Poplar;  LEA;  Evolutionary analyses;  Expression patterns;  Salt stress; 

摘要 Background
The Late Embryogenesis-Abundant (LEA) gene families, which play significant roles in regulation of tolerance to abiotic stresses, widely exist in higher plants. Poplar is a tree species that has important ecological and economic values. But systematic studies on the gene family have not been reported yet in poplar.
Results
On the basis of genome-wide search, we identified 88 LEA genes from Populus trichocarpa and renamed them as PtrLEA. The PtrLEA genes have fewer introns, and their promoters contain more cis-regulatory elements related to abiotic stress tolerance. Our results from comparative genomics indicated that the PtrLEA genes are conserved and homologous to related genes in other species, such as Eucalyptus robusta, Solanum lycopersicum and Arabidopsis. Using RNA-Seq data collected from poplar under two conditions (with and without salt treatment), we detected 24, 22 and 19 differentially expressed genes (DEGs) in roots, stems and leaves, respectively. Then we performed spatiotemporal expression analysis of the four up-regulated DEGs shared by the tissues, constructed gene co-expression-based networks, and investigated gene function annotations.
Conclusion
Lines of evidence indicated that the PtrLEA genes play significant roles in poplar growth and development, as well as in responses to salt stress.

服务人员 孙小满

服务院士 尹伟伦

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