Reshaping of the Arabidopsis thaliana proteome landscape and co-regulation of proteins in development and immunity

编号 040026702

推送时间 20201130

研究领域 森林培育 

年份 2020 

类型 期刊 

语种 英语

标题 Reshaping of the Arabidopsis thaliana proteome landscape and co-regulation of proteins in development and immunity

来源期刊 Molecular Plant

期 第267期

发表时间 20200928

关键词 Arabidopsis;  deep proteomics;  protein co-expression;  proteome;  senescence;  ribosome;  seed proteome;  photosynthesis;  plant immunity;  jasmonate;  IAA;  JOX2;  IAR3;  MYC2; 

摘要 Proteome remodeling is a fundamental adaptive response and proteins in complex and functionally related proteins are often co-expressed. Using a deep sampling strategy we define Arabidopsis thaliana tissue core proteomes at around 10,000 proteins per tissue and absolutely quantify (copy numbers per cell) nearly 16,000 proteins throughout the plant lifecycle. A proteome wide survey of global post translational modification revealed amino acid exchanges pointing to potential conservation of translational infidelity in eukaryotes. Correlation analysis of protein abundance uncovered potentially new tissue and age specific roles of entire signaling modules regulating transcription in photosynthesis, seed development and senescence and abscission. Among others, the data suggest a potential function of RD26 and other NAC transcription factors in seed development related to desiccation tolerance as well as a possible function of Cysteine-rich Receptor-like Kinases (CRKs) as ROS sensors in senescence. All of the components of ribosome biogenesis factor (RBF) complexes were co-expressed in tissue and age specific manners, indicating functional promiscuity in the assembly of these less-studied protein complexes in Arabidopsis. To test our deep proteomics method on basal immunity we treated seedlings with flg22 for 16 hours to characterize proteome architecture in basal immunity in detail. The results were complemented with parallel reaction monitoring (PRM) targeted proteomics, phytohormone, and transcript measurements over 1, 3 and 16 hours of flg22 exposure. We obtained strong evidence of suppression of jasmonate (JA) and JA-Ile levels by deconjugation and hydroxylation via IAA-ALA RESISTANT3 (IAR3) and JASMONATE-INDUCED OXYGENASE 2 (JOX2) under the control of JASMONATE INSENSITIVE 1 (MYC2). This regulatory switch has not been shown in response to flg22 and is another part of the puzzle of the as yet understudied role of JA in pattern triggered immunity. Taken together, the datasets generated in this study present extensive coverage of the Arabidopsis proteome in various biological scenarios, providing a rich resource available to the whole plant science community.

服务人员 孙小满

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