BpTCP7 gene from Betula platyphylla regulates tolerance to salt and drought stress through multiple hormone pathways

编号 040023602

推送时间 20200427

研究领域 森林培育 

年份 2020 

类型 期刊 

语种 英语

标题 BpTCP7 gene from Betula platyphylla regulates tolerance to salt and drought stress through multiple hormone pathways

来源期刊 Plant Cell, Tissue and Organ Culture (PCTOC)

期 第236期

发表时间 20200318

关键词 Betula platyphylla;  CIN subfamily gene;  Gene expression;  Transgenic;  Salt and drought tolerance; 

摘要 TEOSINTE-LIKE1, CYCLOIDEA, and PROLIFERATING CELL FACTOR1 (TCP) genes are plant-specific transcription factors, which play important roles in plant growth and development. The function of CIN subclass of the TCP gene family has not been well revealed. In this investigation, five CIN subfamily members were identified in Betula platyphylla, all of which contained the conservative domains associated with the CIN subclass. Most of the genes were involved in the development of leaves, apical buds, and male inflorescences, especially the BpTCP7 gene. The results of GUS activity assays driven by BpTCP7 promoters confirmed these conclusions. The analysis of the BpTCP7 promoter also showed that the gene was induced by salt and drought stresses, and multiple hormones. Through Agrobacterium-mediated genetic transformation, we obtained four independent birch lines with over-expression of BpTCP7. Moreover, physiological and enzymatic analysis of transgenic lines under salt and drought stress showed that the scavenging effect of reactive oxygen species (ROS) of transgenic lines was improved. Our results provide useful information for revealing the functions of CIN subclasses in the growth and development of B. platyphylla and their applications in scavenging of ROS.

服务人员 孙小满

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