The lncRNA APOLO interacts with the transcription factor WRKY42 to trigger root hair cell expansion in response to cold

编号 040028705

推送时间 20210419

研究领域 森林培育 

年份 2021 

类型 期刊 

语种 英语

标题 The lncRNA APOLO interacts with the transcription factor WRKY42 to trigger root hair cell expansion in response to cold

来源期刊 Molecular Plant

期 第287期

发表时间 20210305

关键词 root hairs;  cell expansion;  long noncoding RNAs;  APOLO;  transcription factors;  RHD6;  43 WRKY42;  cold temperature; 

摘要 Plant long noncoding RNAs (lncRNAs) have emerged as important regulators of chromatin dynamics, impacting on transcriptional programs leading to different developmental outputs. The lncRNA AUXIN REGULATED PROMOTER LOOP (APOLO) directly recognizes multiple independent loci across the Arabidopsis genome and modulates their three-dimensional chromatin conformation, leading to transcriptional shifts. Here, we show that APOLO recognizes the locus encoding the root hair (RH) master regulator ROOT HAIR DEFECTIVE 6 (RHD6) and controls RHD6 transcriptional activity leading to cold-enhanced RH elongation through the consequent activation of the transcription factor gene RHD6-like RSL4. Furthermore, we demonstrate that APOLO interacts with the transcription factor WRKY42 and modulates its binding to the RHD6 promoter. WRKY42 is required for the activation of RHD6 by low temperatures and WRKY42 deregulation impairs cold-induced RH expansion. Collectively, our results indicate that a novel ribonucleoprotein complex involving APOLO and WRKY42 forms a regulatory hub which activates RHD6 by shaping its epigenetic environment and integrates signals governing RH growth and development.

服务人员 孙小满

服务院士 尹伟伦

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