Ectopic expression of GmHP08 enhances resistance of transgenic Arabidopsis toward drought stress

编号 040029105

推送时间 20210517

研究领域 森林培育 

年份 2021 

类型 期刊 

语种 英语

标题 Ectopic expression of GmHP08 enhances resistance of transgenic Arabidopsis toward drought stress

来源期刊 Plant Cell Reports

期 第291期

发表时间 20210316

关键词 Abscisic acid;  Drought resistance;  GmHP08;  Histidine phosphotransfer;  Two-component system; 

摘要 Phosphorelay by two-component system (TCS) is a signal transduction mechanism which has been evolutionarily conserved in both prokaryotic and eukaryotic organisms. Previous studies have provided lines of evidence on the involvement of TCS genes in plant perception and responses to environmental stimuli. In this research, drought-associated functions of GmHP08, a TCS member from soybean (Glycine max L.), were investigated via its ectopic expression in Arabidopsis system. Results from the drought survival assay showed that GmHP08-transgenic plants exhibited higher survival rates compared with their wild-type (WT) counterparts, indicating better drought resistance of the former group. Analyses revealed that the transgenic plants outperformed the WT in various regards, i.e. capability of water retention, prevention of hydrogen peroxide accumulation and enhancement of antioxidant enzymatic activities under water-deficit conditions. Additionally, the expression of stress-marker genes, especially antioxidant enzyme-encoding genes, in the transgenic plants were found greater than that of the WT plants. In contrary, the expression of SAG13 gene, one of the senescence-associated genes, and of several abscisic acid (ABA)-related genes was repressed. Data from this study also revealed that the ectopic expression lines at germination and early seedling development stages were hypersensitive to exogenous ABA treatment. Taken together, our results demonstrated that GmHP08 could play an important role in mediating plant response to drought, possibly via an ABA-dependent manner.

服务人员 孙小满

服务院士 尹伟伦

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