RNA-binding proteins contribute to small RNA loading in plant extracellular vesicles

编号 040028503

推送时间 20210405

研究领域 森林培育 

年份 2021 

类型 期刊 

语种 英语

标题 RNA-binding proteins contribute to small RNA loading in plant extracellular vesicles

来源期刊 Nature Plants

期 第285期

发表时间 20210225

关键词 cell biology;  plant sciences;  extracellular vesicles;  small RNAs (sRNAs); 

摘要 Plants use extracellular vesicles (EVs) to transport small RNAs (sRNAs) into their fungal pathogens and silence fungal virulence-related genes through a phenomenon called ‘cross-kingdom RNAi’. It remains unknown, however, how sRNAs are selectively loaded into EVs. Here, we identified several RNA-binding proteins in Arabidopsis, including Argonaute 1 (AGO1), RNA helicases (RHs) and annexins (ANNs), which are secreted by exosome-like EVs. AGO1, RH11 and RH37 selectively bind to EV-enriched sRNAs but not to non-EV-associated sRNAs, suggesting that they contribute to the selective loading of sRNAs into EVs. Conversely, ANN1 and ANN2 bind to sRNAs non-specifically. The ago1, rh11?rh37 and ann1?ann2 mutants showed reduced secretion of sRNAs in EVs, demonstrating that these RNA-binding proteins play an important role in sRNA loading and/or stabilization in EVs. Furthermore, rh11?rh37 and ann1?ann2 showed increased susceptibility to Botrytis cinerea, suggesting that RH11, RH37, ANN1 and ANN2 positively regulate plant immunity against B. cinerea.

服务人员 孙小满

服务院士 尹伟伦

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