Transcriptome sequencing and gene expression profiling of Populus wutunensis, a natural hybrid, during salinity stress

编号 040026304

推送时间 20201102

研究领域 森林培育 

年份 2020 

类型 期刊 

语种 英语

标题 Transcriptome sequencing and gene expression profiling of Populus wutunensis, a natural hybrid, during salinity stress

来源期刊 Trees

期 第263期

发表时间 20200803

关键词 Differentially expressed gene;  Populus wutunensis;  Salt stress;  Transcription factor; 

摘要 Populus wutunensis, a natural hybrid individual resulting from a cross between Populus?×?xiaozhuanica W. Y. Hsu et Liang, shows salt and drought tolerance and is fast-growing. It is widely cultivated in the Three-North Shelterbelt Forest area in China. To determine the transcriptome changes in P. wutunensis under salt stress, RNA-seq was conducted in salt-treated leaves and non-salt-treated control leaves. A total of 81,188 unigenes, with an average length of 982 bp, were obtained. In salt-treated leaves, 2466 differentially expressed genes were identified that differed from those in control leaves at any time point. Among them, 391 were upregulated and 44 were downregulated during all salt stress time points. They were mainly involved in cellular and metabolic processes, signal transduction, transport, osmotic regulation, and reactive oxygen species (ROS) scavenging. In addition, 2543 transcription factor genes were identified and divided into 60 families. qRT-PCR analysis revealed that the levels of differentially expressed genes were consistent with those from sequencing of the transcriptome under salt stress. These findings will increase our understanding of gene regulation in response to salt stress in P. wutunensis and are beneficial for future research on molecular-assisted breeding.

服务人员 孙小满

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