Targeted A-to-G base editing of chloroplast DNA in plants

编号 040038001

推送时间 20230130

研究领域 森林培育 

年份 2022 

类型 期刊 

语种 英语

标题 Targeted A-to-G base editing of chloroplast DNA in plants

来源期刊 Nature Plants

期 第380期

发表时间 20221201

关键词 A-to-G base editing;  chloroplast DNA;  genes;  CRISPR–Cas9;  DNA editing; 

摘要 Chloroplast DNA (cpDNA) encodes up to 315 (typically, 120–130) genes1, including those for essential components in photosystems I and II and the large subunit of RuBisCo, which catalyses CO2 fixation in plants. Targeted mutagenesis in cpDNA will be broadly useful for studying the functions of these genes in molecular detail and for developing crops and other plants with desired traits. Unfortunately, CRISPR–Cas9 and CRISPR-derived base editors, which enable targeted genetic modifications in nuclear DNA, are not suitable for organellar DNA editing2, owing to the difficulty of delivering guide RNA into organelles. CRISPR-free, protein-only base editors (including DddA-derived cytosine base editors3,4,5,6,7,8 and zinc finger deaminases9), originally developed for mitochondrial DNA editing in mammalian cells, can be used for C-to-T, rather than A-to-G, editing in cpDNA10,11,12. Here we show that heritable homoplasmic A-to-G edits can be induced in cpDNA, leading to phenotypic changes, using transcription activator-like effector-linked deaminases13.

服务人员 孙小满

服务院士 尹伟伦

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