Arabidopsis N6-methyladenosine reader CPSF30-L recognizes FUE signal to control polyadenylation site choice in liquid-like nuclear body

编号 040027905

推送时间 20210222

研究领域 森林培育 

年份 2021 

类型 期刊 

语种 英语

标题 Arabidopsis N6-methyladenosine reader CPSF30-L recognizes FUE signal to control polyadenylation site choice in liquid-like nuclear body

来源期刊 Molecular Plant

期 第279期

发表时间 20210127

关键词 Epitranscriptomics;  N6-methyladenosine (m6A);  m6A reader;  CPSF30-L;  phase separation;  Alternative polyadenylation (APA); 

摘要 The biological functions of epitranscriptomic modification N 6-methyladenosine (m 6A) in plants have not been fully understood. CPSF30-L is a predominant isoform of polyadenylation factor CPSF30 and consists of CPSF30-S and m 6A-binding YTH domain. Little is known about the biological roles of CPSF30-L and the molecular mechanism underlying its m 6A binding function in alternative polyadenylation (APA). Here we characterized CPSF30-L as an Arabidopsis m 6A reader whose m 6A binding function is required for floral transition and abscisic acid (ABA) response. We found the m 6A binding activity enhances the formation of liquid-like CPSF30-L nuclear bodies, where CPSF30-L mainly recognizes m 6A-modified far upstream element (FUE) to control polyadenylation site choice. Disruption of CPSF30-L lengthens 3' untranslated region (3' UTR) of three CPSF30-L binding transcripts related with phenotypes, thereby accelerating their mRNA degradation and leading to late flowering and ABA hypersensitivity. This study uncovers a new molecular mechanism for m 6A-driven phase separation and polyadenylation in plants.

服务人员 孙小满

PDF文件 浏览全文