Designs, applications, and limitations of genetically encoded fluorescent sensors to explore plant biology

编号 040031505

推送时间 20211101

研究领域 森林培育 

年份 2021 

类型 期刊 

语种 英语

标题 Designs, applications, and limitations of genetically encoded fluorescent sensors to explore plant biology

来源期刊 Plant Physiology

期 第315期

发表时间 20210830

关键词 plant biology;  spatial dynamics;  imaging systems; 

摘要 The understanding of signaling and metabolic processes in multicellular organisms requires knowledge of the spatial dynamics of small molecules and the activities of enzymes, transporters, and other proteins in vivo, as well as biophysical parameters inside cells and across tissues. The cellular distribution of receptors, ligands, and activation state must be integrated with information about the cellular distribution of metabolites in relation to metabolic fluxes and signaling dynamics in order to achieve the promise of in vivo biochemistry. Genetically encoded sensors are engineered fluorescent proteins that have been developed for a wide range of small molecules, such as ions and metabolites, or to report biophysical processes, such as transmembrane voltage or tension. First steps have been taken to monitor the activity of transporters in vivo. Advancements in imaging technologies and specimen handling and stimulation have enabled researchers in plant sciences to implement sensor technologies in intact plants. Here, we provide a brief history of the development of genetically encoded sensors and an overview of the types of sensors available for quantifying and visualizing ion and metabolite distribution and dynamics. We further discuss the pros and cons of specific sensor designs, imaging systems, and sample manipulations, provide advice on the choice of technology, and give an outlook into future developments.

服务人员 孙小满

服务院士 尹伟伦

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