Phytochrome B induces intron retention and translational inhibition of PHYTOCHROME-INTERACTING FACTOR 3

编号 040021802

推送时间 20191223

研究领域 森林培育 

年份 2019 

类型 期刊 

语种 英语

标题 Phytochrome B induces intron retention and translational inhibition of PHYTOCHROME-INTERACTING FACTOR 3

来源期刊 Plant Physiology

期 第218期

发表时间 20191105

关键词 phytochrome B;  PIF3;  translational regulation;  alternative splicing;  intron
24 retention;  uORF;  photomorphogenesis;  short day;  diurnal cycle;  light regulation; 
25 Arabidopsis; 

摘要 The phytochrome B (phyB) photoreceptor stimulates light responses in plants in part by inactivating repressors of light responses, such as PHYTOCHROME-INTERACTING FACTOR3 (PIF3). Activated phyB inhibits PIF3 by rapid protein degradation and decreased transcription. PIF3 protein degradation is mediated by EIN3-BINDING F-BOX PROTEIN (EBF) and LIGHT-RESPONSE BTB (LRB) E3 ligases, the latter of which simultaneously targets phyB for degradation. In this study, we show PIF3 levels are additionally regulated by alternative splicing and protein translation in Arabidopsis (Arabidopsis thaliana). Overaccumulation of photo-activated phyB, which occurs in the mutant defective for LRB genes under continuous red light, induces a specific alternative splicing of PIF3 that results in retention of an intron in the 5' untranslated region (5' UTR) of PIF3 mRNA. In turn, the upstream open reading frames (uORFs) contained within this intron inhibit PIF3 protein synthesis. The phyB-dependent alternative splicing of PIF3 is diurnally regulated under the short-day light cycle. We hypothesize this reversible regulatory mechanism may be utilized to fine tune the level of PIF3 protein in light-grown plants and may contribute to the oscillation of PIF3 protein abundance under the short-day environment.

服务人员 孙小满

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