Convergence of sphingolipid desaturation across over 500 million years of plant evolution

编号 040028003

推送时间 20210301

研究领域 森林培育 

年份 2021 

类型 期刊 

语种 英语

标题 Convergence of sphingolipid desaturation across over 500 million years of plant evolution

来源期刊 Nature Plants

期 第280期

发表时间 20210125

关键词 plant evolution;  low temperatures;  Physcomitrium (Physcomitrella) patens;  PpSFD; 

摘要 or plants, acclimation to low temperatures is fundamental to survival. This process involves the modification of lipids to maintain membrane fluidity. We previously identified a new cold-induced putative desaturase in Physcomitrium (Physcomitrella) patens. Lipid profiles of null mutants of this gene lack sphingolipids containing monounsaturated C24 fatty acids, classifying the new protein as sphingolipid fatty acid denaturase (PpSFD). PpSFD mutants showed a cold-sensitive phenotype as well as higher susceptibility to the oomycete Pythium, assigning functions in stress tolerance for PpSFD. Ectopic expression of PpSFD in the Atads2.1 (acyl coenzyme A desaturase-like 2) Arabidopsis thaliana mutant functionally complemented its cold-sensitive phenotype. While these two enzymes catalyse a similar reaction, their evolutionary origin is clearly different since AtADS2 is a methyl-end desaturase whereas PpSFD is a cytochrome b5 fusion desaturase. Altogether, we suggest that adjustment of membrane fluidity evolved independently in mosses and seed plants, which diverged more than 500?million years ago.

服务人员 孙小满

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