Cold-Induced CBF-PIF3 Interaction Enhances Freezing Tolerance by Stabilizing the phyB Thermosensor in Arabidopsis

编号 040024403

推送时间 20200622

研究领域 森林培育 

年份 2020 

类型 期刊 

语种 英语

标题 Cold-Induced CBF-PIF3 Interaction Enhances Freezing Tolerance by Stabilizing the phyB Thermosensor in Arabidopsis

来源期刊 Molecular Plant

期 第244期

发表时间 20200417

关键词 CBFs;  PIFs;  phyB;  freezing tolerance;  Arabidopsis; 

摘要 Growth inhibition and cold acclimation strategies help plants withstand cold stress which adversely affects growth and survival. PHYTOCHROME B (phyB) regulates plant growth through perceiving both light and ambient temperature signals. However, the mechanism by which phyB mediates the plant response to cold stress remains elusive. Here, we show that the key transcription factors mediating cold acclimation, C-REPEAT BINDING FACTORs (CBFs), interact with PHYTOCHROME-INTERACTING FACTOR3 (PIF3) under cold stress, thus attenuating the mutually assured destruction of PIF3-phyB. Cold-stabilized phyB acts downstream of CBFs to positively regulate freezing tolerance by modulating the expression of stress-responsive and growth-related genes. Consistently, phyB mutants exhibited a freezing-sensitive phenotype, whereas phyB-overexpression transgenic plants displayed enhanced freezing tolerance. Further analysis showed that PIF1, PIF4, and PIF5 proteins, all of which negatively regulate plant freezing tolerance, were destabilized by cold stress in a phytochrome-dependent manner. This study thus uncovers that CBFs-PIF3-phyB serves as an important regulatory module for regulating plant response to cold stress.

服务人员 孙小满

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