Mutagenomics: The Future of Genetic Screens

编号 040027204

推送时间 20210104

研究领域 森林培育 

年份 2021 

类型 期刊 

语种 英语

标题 Mutagenomics: The Future of Genetic Screens

来源期刊 Plant Physiology

期 第272期

发表时间 20201127

关键词 mutagenomics;  geneticscreens;  plant growth;  Arabidopsis; 

摘要 Genetic screens are a remarkable way of identifying mutations affecting various aspects of plant growth and development. Arabidopsis (Arabidopsis thaliana) (T)-DNA insertion lines enable rapid identification of mutant genes but are tedious to create in a mutant background of choice. Chemical mutagens such as ethyl methanesulfonate (EMS) create hundreds of allelic variants in any background of choice and potentially avoid lethality of null alleles. However, cloning these variants can be challenging. Additionally, EMS mutagenesis causes many single-nucleotide polymorphisms (SNPs) that are not causative for the mutation of interest. Backcrossing allows the identification of causative mutations but is labor intensive. High-throughput sequencing has facilitated the development of several pipelines that involve cloning genes corresponding to allelic variants, thereby reducing the need for extensive backcrossing (Michelmore et al., 1991; Cluis et al., 2004; Austin et al., 2011; Nordstr?m et al., 2013). Although powerful, only a limited number of lines are generally screened by these methods. For many pathways of interest, prior genetic screens have already identified multiple genes. The use of enhancer/suppressor screens combined with high-throughput analysis of identified mutations has the potential to identify novel elements in a pathway of interest.

服务人员 孙小满

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