A comprehensive fluorescent sensor for spatiotemporal cell cycle analysis in Arabidopsis

编号 040026602

推送时间 20201123

研究领域 森林培育 

年份 2020 

类型 期刊 

语种 英语

标题 A comprehensive fluorescent sensor for spatiotemporal cell cycle analysis in Arabidopsis

来源期刊 Nature Plant

期 第266期

发表时间 20200928

关键词 fluorescence imaging;  plant development;  Arabidopsis;  cell cycle; 

摘要 Assessing cell proliferation dynamics is crucial to understand the spatiotemporal control of organogenesis. Here we have generated a versatile fluorescent sensor, PlaCCI (plant cell cycle indicator) on the basis of the expression of CDT1a-CFP, H3.1-mCherry and CYCB1;1-YFP, that identifies cell cycle phases in Arabidopsis thaliana. This tool works in a variety of organs, and all markers and the antibiotic resistance are expressed from a single cassette, facilitating the selection in mutant backgrounds. We also show the robustness of PlaCCI line in live-imaging experiments to follow and quantify cell cycle phase progression.

服务人员 孙小满

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