Deep conservation of cis-element variants regulating plant hormonal responses

编号 040020903

推送时间 20191021

研究领域 森林培育 

年份 2019 

类型 期刊 

语种 英语

标题 Deep conservation of cis-element variants regulating plant hormonal responses

来源期刊 The Plant Cell

期 第209期

发表时间 20190829

关键词 Cis-element variants;  hormonal response;  transcription factors(TFs);  response elements; 

摘要 Phytohormones regulate many aspects of plant life by activating transcription factors (TF) that bind sequence-specific response elements (RE) in regulatory regions of target genes. Despite their short length, REs are degenerate with a core of just 3-4bp. This degeneracy is paradoxical, as it reduces specificity and REs are extremely common in the genome. To study whether RE degeneracy might serve a biological function we developed an algorithm for the detection of regulatory sequence conservation and applied it to phytohormones REs in 45 angiosperms. Surprisingly, we found that specific RE variants are highly conserved in core hormone response genes. Experimental evidence showed that specific variants act to regulate the magnitude and spatial profile of hormonal response in Arabidopsis and tomato. Our results suggest that hormone-regulated TFs bind a spectrum of REs, each coding for a distinct transcriptional response profile. Our approach has implications for precise genome editing and for rational promoter design.

服务人员 孙小满

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