Single-cell transcriptomics sheds light on the identity and metabolism of developing leaf cells

编号 040033702

推送时间 20220404

研究领域 森林培育 

年份 2021 

类型 期刊 

语种 英语

标题 Single-cell transcriptomics sheds light on the identity and metabolism of developing leaf cells

来源期刊 Plant Physiology

期 第337期

发表时间 20211023

关键词 Single-cell；transcriptomics photosynthetic；Arabidopsis thaliana；high-throughput transcriptome metabolic；stress-related; 

摘要 As the main photosynthetic instruments of vascular plants, leaves are crucial and complex plant organs. A strict organization of leaf mesophyll and epidermal cell layers orchestrates photosynthesis and gas exchange. In addition, water and nutrients for leaf growth are transported through the vascular tissue. To establish the single-cell transcriptomic landscape of these different leaf tissues, we performed high-throughput transcriptome sequencing of individual cells isolated from young leaves of Arabidopsis (Arabidopsis thaliana) seedlings grown in two different environmental conditions. The detection of approximately 19,000 different transcripts in over 1,800 high-quality leaf cells revealed 14 cell populations composing the young, differentiating leaf. Besides the cell populations comprising the core leaf tissues, we identified subpopulations with a distinct identity or metabolic activity. In addition, we proposed cell-type-specific markers for each of these populations. Finally, an intuitive web tool allows for browsing the presented dataset. Our data present insights on how the different cell populations constituting a developing leaf are connected via developmental, metabolic, or stress-related trajectories.

服务人员 孙小满

服务院士 尹伟伦

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