Coordination of plant growth and abiotic stress responses by Tryptophan Synthase β Subunit1 through modulating tryptophan and ABA homeostasis in Arabidopsis

编号 040035102

推送时间 20220711

研究领域 森林培育 

年份 2022 

类型 期刊 

语种 英语

标题 Coordination of plant growth and abiotic stress responses by Tryptophan Synthase β Subunit1 through modulating tryptophan and ABA homeostasis in Arabidopsis

来源期刊 Molecular Plant

期 第351期

发表时间 20220428

关键词 Arabidopsis;  ABA;  abiotic stress;  Tryptophan Synthase β Subunit1;  tryptophan;  stress responses;  drought;  salinity; 

摘要 To adjust to changing environments, plants have evolved elaborate regulatory mechanisms balancing their growth with their stress responses. It is currently unknown whether and how the growth-related hormone auxin and stress hormone abscisic acid (ABA) are coordinated in this trade-off. Here, we show that TRYPTOPHAN (Trp) SYNTHASE β SUBUNIT1 (TSB1) is involved in the coordination of Trp and ABA, thereby affecting plant growth and abiotic stress responses. Plants experiencing high salinity or drought display reduced TSB1 expression, resulting in decreased tryptophan and auxin accumulation, and thus reduced growth. In comparison with the wild type, the amiR-TSB1 lines and TSB1 mutants exhibited repressed growth under non-stress conditions but have enhanced ABA accumulation and stress tolerance when subjected to salt or drought stress. Furthermore, TSB1 interacts with and inhibits β-GLUCOSIDASE1 (BG1), which hydrolyses glucose-conjugated ABA into active ABA. The mutation of BG1 in the amiR-TSB1 lines compromised their increased ABA accumulation and enhanced stress tolerance. Moreover, stress-induced H2O2 interrupted the interaction between TSB1 and BG1 by sulfenylating cysteine-308 of TSB1, relieving the TSB1-mediated inhibition of BG1 activity. Taken together, we revealed that TSB1 is a key coordinator of plant growth and stress responses through balancing Trp and ABA homeostasis.

服务人员 孙小满

服务院士 尹伟伦

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