eDNA宏条形码技术在城市生物多样性监测中的适用性与展望

编号 lyqk011089

中文标题 eDNA宏条形码技术在城市生物多样性监测中的适用性与展望

作者 廖晨阳  杨明乐  高庆  毛颖  冯可心 

作者单位 1. 四川大学建筑与环境学院 成都 610065;
2. 四川省城乡建设研究院 成都 610041

期刊名称 中国城市林业 

年份 2023 

卷号 21

期号 3

栏目名称 综述 

中文摘要 城市生物监测是城市生物多样性研究的重要内容之一,但由于城市环境和生物习性的复杂性,传统生物监测方法的应用受到诸多制约。eDNA宏条形码技术能够便捷地从环境中提取DNA片段并扩增与测序,从而快速准确地鉴定生物群落物种成分,近年来已成功应用于自然环境生物监测。文章基于eDNA宏条形码技术的主要优势和特点,探讨该技术应用于城市环境中食物链与生物食性、疫源生物、入侵生物及濒危物种、隐遁生物、特殊区域生物以及大气生物源性污染的监测等方面的适用性,归纳其存在的局限性,并对其发展趋势提出展望。

关键词 eDNA  生态监测  城市生态  生物多样性  物种识别 

基金项目 国家自然科学基金(31400195;31700176);四川大学研究生培养教育创新改革项目(2023HXKC012)

英文标题 Applicability and Prospect of Environmental DNA Metabarcoding Technology for Urban Biodiversity Monitoring

作者英文名 Liao Chenyang, Yang Mingle, Gao Qing, Mao Ying, Feng Kexin

单位英文名 1. College of Architecture and Environment, Sichuan University, Chengdu 610065, China;
2. Sichuan Institute of Urban and Rural Construction, Chengdu 610041, China

英文摘要 Urban biomonitoring is an important part of the research on urban biodiversity, but the traditional biomonitoring methods is very restricted in its application due to the complexity in urban environment and biological habits. Environmental DNA metabarcoding technology can easily and quickly identify species components of biological community by amplifying and sequencing the DNA fragments extracted from the environment, which makes it widely used in biological monitoring of natural environment in recent years. Based on the advantages and characteristics of eDNA metabarcoding technology, this paper discusses its applicability in the monitoring of food chain and animal feeding, epidemic source organisms, invasive organisms, endangered species, reclusive organisms, organisms in special areas and air biogenic pollution in urban environment. In view of the limitations of this technology, the authors propose the prospect of its development trend.

英文关键词 eDNA;ecological monitoring;urban eco-environment;biodiversity;species identification

起始页码 167

截止页码 173

投稿时间 2023/4/10

作者简介 廖晨阳(1982-),男,博士,副教授,研究方向为城乡生物多样性与植物应用。E-mail:chenyangliao@scu.edu.cn

通讯作者介绍 冯可心(1983-),男,硕士,高级工程师,研究方向为城乡规划与遗产保护。E-mail:kexin20023075@qq.com

E-mail kexin20023075@qq.com

DOI 10.12169/zgcsly.2023.04.10.0002

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