编号
lyqk007916
中文标题
林木体细胞胚胎发生机理研究进展
作者单位
贵州大学林学院, 贵阳 550025
期刊名称
世界林业研究
年份
2019
卷号
32
期号
5
栏目编号
1
栏目名称
专题论述
中文摘要
体细胞胚胎发生技术能够实现优良林木品种的规模化繁育及优良性状保留,为林木扩繁、种质资源保存、遗传改良和机理研究等提供材料。但其仍存在诱导率低、褐化现象严重、愈伤分化困难和再生植株生长较弱等问题。文中从生理生化学、细胞生物学和分子生物学3个方面阐述体胚发生机理,包括内源激素含量、细胞程序性死亡和体胚发生相关基因等,并在当前研究基础上对未来林木体胚发生深入研究进行展望,旨在为建立高效林木体胚发生体系及其机理研究提供参考。
基金项目
国家自然科学基金“花榈木根瘤菌多样性及幼苗形成调控因素研究”(31460193);贵州省高层次创新型人才培养计划“百”层次人才项目“贵州珍贵树种栽培生理生态及精准化育苗技术研究”(黔科合平台人才[2016]5661);贵州省林业厅项目“贵州优质乡土阔叶树种培育与示范”[黔林科合(2010)重大02]。
英文标题
Research Progress on Mechanism of Somatic Embryogenesis in Forest Trees
作者英文名
Wu Gaoyin, Wei Xiaoli, Wang Xiao, Liu Yingying, Chen Shengqun
单位英文名
College of Forestry, Guizhou University, Guiyang 550025, China
英文摘要
Somatic embryogenesis (SE) is an effective way for large-scale breeding and good traits retention of superior tree species, which provides materials for tree propagation, germplasm conservation, genetic improvement and mechanism research. However, SE still has problems such as low induction rate, severe browning, difficulty of callus differentiation and weak growth of regenerated plants. This paper explains the mechanism of SE from physiological biochemistry, cell biology and molecular biology, including endogenous hormone content, programmed cell death (PCD) and key genes of somatic embryogenesis, and put forward the future development direction of forest tree SE based on current researches, which is aimed at providing references for establishing an efficient system of forest tree SE and related mechanism research.
英文关键词
somatic embryogenesis;physiological biochemistry;cell biology;molecular biology
起始页码
9
截止页码
14
投稿时间
2018/11/13
最后修改时间
2019/5/26
作者简介
吴高殷,男,博士研究生,研究方向为种苗繁育,E-mail:wugaoyin1234@163.com。
通讯作者介绍
韦小丽,教授,博士生导师,研究方向为种苗繁育,E-mail:gdwxl-69@126.com。
E-mail
韦小丽,教授,博士生导师,研究方向为种苗繁育,E-mail:gdwxl-69@126.com。
分类号
S718.43;S718.46
DOI
10.13348/j.cnki.sjlyyj.2019.0046.y
参考文献
[1] FEHÉR A. Somatic embryogenesis:stress-induced remodeling of plant cell fate[J]. Gene Regulatory Mechanisms,2015,1849(4):385-402.
[2] 崔凯荣. 植物体细胞胚发生的分子生物学[M]. 北京:科学出版社,2000.
[3] KLUBICOVÁ K,UVÁCKOVÁ L,DANCHENKO M,et al. Insights into the early stage of Pinus nigra Arn. somatic embryogenesis using discovery proteomics[J]. Journal of Proteomics,2017,169:99-111.
[4] ARDIYANI F. Morphological characterization and identification of Coffea liberica callus of somatic embryogenesis[J]. Pelita Perkebunan,2015,31(2):81-89.
[5] ARNOLD S V,SABALA I,BOZHKOV P,et al. Developmental pathways of somatic embryogenesis[J]. Plant Cell Tissue and Organ Culture,2002,69(3):233-249.
[6] 齐力旺. 华北落叶松体细胞胚胎发生与遗传转化系统建立的研究[D]. 北京:中国林业科学研究院,2000.
[7] 张建伟. 粗枝云杉体胚发生和萌发前的干化调控机制[D]. 北京:中国林业科学研究院,2014.
[8] 陈金慧,施季森,诸葛强,等. 杂交鹅掌楸体细胞胚胎发生研究[J]. 林业科学,2003,39(4):49-53.
[9] HOU J,WU Y,SHEN Y,et al. Plant regeneration through somatic embryogenesis and shoot organogenesis from immature zygotic embryos of Sapium sebiferum Roxb[J]. Scientia Horticulturae,2015,197:218-225.
[10] YASEEN M,AHMAD T,SABLOK G,et al. Review:role of carbon sources for in vitro plant growth and development[J]. Molecular Biology Reports,2013,40(4):2837-2849.
[11] 李茜. 白皮松体细胞胚胎发生的研究[D]. 陕西杨凌:西北农林科技大学,2006.
[12] 江荣翠. 滇楸体细胞胚胎发生及其机理研究[D]. 南京:南京林业大学,2010.
[13] 王高. 红松体细胞胚胎发生及超低温保存技术研究[D]. 上海:上海交通大学,2009.
[14] 李香竹. 刺五加体细胞胚胎发育同步化控制[D]. 长春:吉林农业大学,2012.
[15] 顾玉红. 文冠果体细胞胚胎发生及形态建军成机理的研究[D]. 北京:北京林业大学,2005.
[16] 陈少瑜. 丽江云杉体胚发生体系优化及体胚发育过程蛋白质组分析[D]. 昆明:云南大学,2010.
[17] 刘华英. 柑橘体细胞胚发生的细胞学及生理生化特性研究[D]. 长沙:湖南农业大学,2003.
[18] 刘翠琼,尹伟伦,夏新莉. 中国沙棘和北美红杉体细胞胚胎诱导及其组织细胞学的研究[M]. 北京:中国环境科学出版社,2009.
[19] 刘春苹,杨玲,沈海龙. 植物体细胞胚胎发生发育过程中的细胞程序性死亡[J]. 安徽农业科学,2009,37(28):3464-3467.
[20] 孙张晗,樊怀福,杜长霞,等. 盐胁迫对黄瓜幼苗叶片、韧皮部渗出液和根系抗氧化酶同工酶表达的影响[J]. 浙江农林大学学报,2016,33(4):652-657.
[21] 李青粉. 川西云杉早期体细胞胚胎发生机理研究[D]. 北京:中国林业科学研究院,2014.
[22] 黄素华. 荔枝体细胞胚胎发生过程中遗传变异的研究[D]. 福州:福建农林大学,2002.
[23] 王长兰,张青,魏文桃,等. 楸树体细胞胚胎发生过程中4种同工酶分析[J]. 基因组学与应用生物学,2016,35(11):3122-3127.
[24] 程文翰. 棉花(Gossypium hirsutum L.)体细胞胚胎发生的生理及分子机制研究[D]. 新疆石河子:石河子大学,2016.
[25] 赖钟雄,陈春玲. 龙眼体细胞胚胎发生过程中的内源激素变化[J]. 热带作物学报,2002,32(2):41-47.
[26] 车建美,赖钟雄,赖呈纯,等. 荔枝体细胞胚胎发生早期的3种内源激素含量变化[J]. 热带作物学报,2005,26(2):55-61.
[27] 黄道斌. 茶树体胚发生及其内源激素变化与apx基因表达分析[D]. 福州:福建农林大学,2009.
[28] 李哲馨. 过表达LaMIR166a在落叶松ESMs成胚发育及其萌发过程中的功能研究[D]. 北京:中国林业科学研究院,2017.
[29] VALES T,FENG X,GE L,et al. Improved somatic embryo maturation in loblolly pine by monitoring ABA-responsive gene expression[J]. Plant Cell Reports,2007,26(2):133-143.
[30] 谭德冠. 巴西橡胶树体胚发生的改良及乳管分化研究[D]. 海口:海南大学,2011.
[31] KUMAR V,GIRIDHAR P,CHANDRASHEKAR A,et al. Polyamines influence morphogenesis and caffeine biosynthesis in in vitro cultures of Coffea canephora P. ex Fr.[J]. Acta Physiologiae Plantarum,2008,30(2):217-223.
[32] ROBIE C A,MINOCHA S C. Polyamines and somatic embryogenesis in carrot:I.the effects of difluoromethylornithine and difluoromethylarginine[J]. Plant Science,1989,65(1):45-54.
[33] PUGAHERMIDA M I,GALLARDO M,RODRIGUEZGACIO M C,et al. Polyamine contents,ethylene synthesis,and BrACO2 expression during turnip germination[J]. Biologia Plantarum,2006,50(4):574-580.
[34] KAKKAR R K,NAGAR P K,AHUJA P S,et al. Polyamines and plant morphogenesis[J]. Biologia Plantarum,2000,43(1):1-11.
[35] HILL R D,HUANG S,STASOLLA C. Hemoglobins,programmed cell death and somatic embryogenesis[J]. Plant Science,2013,211(3):35-41.
[36] SMERTENKO A,BOZHKOV P V. Somatic embryogenesis:life and death processes during apical-basal patterning[J]. Journal of Experimental Botany,2014,65(5):1343-1360.
[37] YEUNG E C,MEINKE D W. Embryogenesis in angiosperms:development of the suspensor[J]. Plant Cell,1993,5(10):1371-1381.
[38] VAN DOORN W G,BEERS E P,DANGL J L,et al. Morphological classification of plant cell deaths[J]. Cell Death and Differentiation,2011,18(8):1241-1246.
[39] 吴家和,张献龙,聂以春. 棉花体细胞增殖和胚胎发生中的细胞程序性死亡[J]. 植物生理与分子生物学学报,2003,29(6):515-520.
[40] ARAVINDAKUMAR C T,DE L M,CEULEMANS J. Nitric oxide induces Zn2+ release from metallothionein by destroying zinc-sulfur clusters without concomitant formation of S-nitrosothiol[J]. Biochemical Journal,1999,344(1):253-258.
[41] 王晶. 水曲柳体胚发生技术优化及H2O2和NO作用分析[D]. 哈尔滨:东北林业大学,2015.
[42] 邢更妹,李杉,崔凯荣,等. 植物体细胞胚发生中抗氧化系统代谢动态和程序性细胞死亡[J]. 生命科学,2000,12(5):214-216.
[43] He Y F,LI B Z,LI Z,et al. Tet-mediated formation of 5-carboxylcytosine and its excision by TDG in mammalian DNA[J]. Science,2011,333(6047):1303-1307.
[44] 李辉亮,郭冬,彭世清. 巴西橡胶树体细胞胚发生过程中DNA甲基化分析[J]. 热带亚热带植物学报,2015,23(5):527-533.
[45] 魏华丽. 落叶松体细胞胚胎发生过程中DNA甲基化分析及MET1、DDM1克隆研究[D]. 北京:中国林业科学研究院,2010.
[46] 姚培娟,李际红,亓晓,等. 欧石楠体细胞胚发生过程中的DNA甲基化[J]. 植物生理学报,2013,49(12):1413-1420.
[47] HAO Y,DENG X. Stress treatments and DNA methylation affected the somatic embryogenesis of citrus callus[J]. Acta Botanica Sinica,2002,44(6):673-677.
[48] 白玉,陈晓慧,谢礼洋,等. 龙眼胚性愈伤组织DRM1基因的克隆及其定位与表达分析[J]. 西北植物学报,2017,37(11):2097-2105.
[49] 魏丕伟. 杂交鹅掌楸体细胞胚胎发生标志基因克隆及表达分析[D]. 南京:南京林业大学,2009.
[50] SCHMIDT E D,GUZZO F,TOONEN M A,et al. A leucine-rich repeat containing receptor-like kinase marks somatic plant cells competent to form embryos[J]. Development,1997,124(10):2049-2062.
[51] MDEO S,ROMANO E,KSC Y,et al. Characterisation of the cacao somatic embryogenesis receptor-like kinase (SERK) gene expressed during somatic embryogenesis[J]. Plant Science,2005,168(3):723-729.
[52] RUPPS A,RASCHKE J,RüMMLER M,et al. Identification of putative homologs of Larix decidua to Babyboom (BBM),LEAFY COTYLEDON1(LEC1),WUSCHEL-related HOMEOBOX2 (WOX2) and SOMATIC EMBRYOGENESIS RECEPTOR-like KINASE (SERK) during somatic embryogenesis[J]. Planta,2016,243(2):473-488.
[53] MA J,HE Y H,HU Z Y,et al. Histological analysis of somatic embryogenesis in pineapple:AcSERK1 and its expression validation under stress conditions[J]. Journal of Plant Biochemistry and Biotechnology,2016,25(1):49-55.
[54] MAYER K F X,SCHOOF H,HAECKER A,et al. Role of WUSCHEL in regulating stem cell fate in the Arabidopsis shoot meristem[J]. Cell,1998,95(6):805-815.
[55] ARROYO-HERRERA A,GONZALEZ A K,MOO R C,et al. Expression of WUSCHEL in Coffea canephora causes ectopic morphogenesis and increases somatic embryogenesis[J]. Plant Cell Tissue and Organ Culture,2008,94(2):171-180.
[56] NICCAN G I,LÓPEZTORRES A,BARREDOPOOL F,et al. New insights into somatic embryogenesis:LEAFY COTYLEDON1,BABY BOOM1and WUSCHEL-RELATED HOMEOBOX4 are epigenetically regulated in Coffea canephora[J]. Plos One,2013,8(8):e72160. DOI:10.1371/journal.pone.0072160.
[57] GUO F,LIU C,XIA H,et al. Induced expression of AtLEC1and AtLEC2differentially promotes somatic embryogenesis in transgenic tobacco plants[J]. Plos One,2013,8(8):1-7.
[58] BOUTILIER K,OFFRINGA R,SHARMA V K,et al. Ectopic expression of BABY BOOM triggers a conversion from vegetative to embryonic growth[J]. Plant Cell,2002,14(8):1737-1749.
[59] LU D,WEI W,ZHOU W,et al. Establishment of a somatic embryo regeneration system and expression analysis of somatic embryogenesis-related genes in Chinese chestnut (Castanea mollissima Blume)[J]. Plant Cell Tissue and Organ Culture,2017,130(3):1-16.
PDF全文
浏览全文