The Fate of Mitochondria in lbex-hirus Reconstructed Early Embryos

编号 zgly0000334564

文献类型 期刊论文

文献题名 The Fate of Mitochondria in lbex-hirus Reconstructed Early Embryos

作者 YanJIANG  Shu-ZhenLIU  Yan-LingZHANG  Man-XiJIANG  Qing-YuanSUN  Da-YuanCHEN 

作者单位 StateKeyLaboratoryofReproductiveBiology  InstituteofZoology  theChineseAcademyofSciences  Beijing100080  China 

母体文献 生物化学与生物物理学报 

年卷期 2004,36(5)

页码 371-374

年份 2004 

分类号 Q959.842  Q78 

关键词 线粒体  重建  野山羊  胚胎  克隆  PCR 

文摘内容 Inter-species nuclear transfer could be used to preserve North Goat (Capra ibex), an endangered species. We established the culture conditions for ibex-hirus reconstructed embryos and optimized the method for DNA extractions of a single cell and early cloned embryo. By using mitochondria-specific probes of ibex and hirus respectively we found that mitochondria of donor cells can co-exist with recipients in 1-cell and 2-cell stages of the reconstructed embryos but not in the following developmental stages。