Study on Mutagenic Breeding of Bacillus Subtilis and Propertiesof Its Antifungal Substances

编号 zgly0000349170

文献类型 期刊论文

文献题名 Study on Mutagenic Breeding of Bacillus Subtilis and Propertiesof Its Antifungal Substances

作者 刘静  姚建铭 

作者单位 KeyLaboratoryofIonBeamBioengineering  ChineseAcademyofSciences  Hefei 

母体文献 Plasma Science & technology;等离子体科学和技术: 英文版 

年卷期 2004,6(4)

页码 2433-2436

年份 2004 

分类号 TQ453.5  TL99 

关键词 离子束注入  抗真菌物质  诱变繁殖  杆菌  生物杀虫剂 

文摘内容 Bacillus subtitles JA isolated by our laboratory produced a large amount of antifungal substances, which had strong inhibitory activity against various plant pathogenic fungi,such as Rhizoctonia solani, l?usarium graminearum and so on. Ion beam implantation as a new mutagenic methods was applied in our studay. After B. subtitles JA was implanted by N^+ ions,a strain designated as B. subtitles JA-026 was screened and obtained, which had a higher ability to produce those antifungal substances. A series of experiments indicated that the antifungal substances were thermostable and partially sensitive to proteinases K and tryproteinase. When the fermentating broth was fractionated with ammonium sulphate of a final saturation of 70%,the precipitate-enhanced inhibitory activity while the supernatant lost this activity. It appeared that the antifungal substances were likely to be protein。