ERAD-related E2 and E3 enzymes modulate the drought response by regulating the stability of PIP2 aquaporins

编号 040031205

推送时间 20211011

研究领域 森林培育 

年份 2021 

类型 期刊 

语种 英语

标题 ERAD-related E2 and E3 enzymes modulate the drought response by regulating the stability of PIP2 aquaporins

来源期刊 The Plant cell

期 第312期

发表时间 20210520

关键词 Endoplasmic reticulum-associated degradation (ERAD);  drought response;  drought tolerance;  UBC32;  Lys276;  PIP2 aquaporins; 

摘要 Endoplasmic reticulum-associated degradation (ERAD) is known to regulate plant responses to diverse stresses, yet its underlying molecular mechanisms and links to various stress signaling pathways are poorly understood. Here, we show that the ERAD component ubiquitin-conjugating enzyme UBC32 positively regulates drought tolerance in Arabidopsis thaliana by targeting the aquaporins PIP2;1 and PIP2;2 for degradation. Furthermore, we demonstrate that the RING-type ligase Rma1 acts together with UBC32 and that the E2 activity of UBC32 is essential for the ubiquitination of Rma1. This complex ubiquitinates a phosphorylated form of PIP2;1 at Lys276 to promote its degradation, thereby enhancing plant drought tolerance. Extending these molecular insights into crops, we show that overexpression of Arabidopsis UBC32 also improves drought tolerance in rice (Oryza sativa). Thus, beyond uncovering the molecular basis of an ERAD-regulated stress response, our study suggests multiple potential strategies for engineering crops with improved drought tolerance.

服务人员 孙小满

服务院士 尹伟伦

PDF文件 浏览全文