Hydrogen Sulfide Positively Regulates Abscisic Acid Signaling through Persulfidation of SnRK2.6 in Guard Cells

编号 040023403

推送时间 20200413

研究领域 森林培育 

年份 2020 

类型 期刊 

语种 英语

标题 Hydrogen Sulfide Positively Regulates Abscisic Acid Signaling through Persulfidation of SnRK2.6 in Guard Cells

来源期刊 MOLECULAR PLANT

期 第234期

发表时间 20200117

关键词 H2S;  ABA;  Persulfidation;  Ca2+;  Stomatal closure;  Drought; 

摘要 The phytohormone abscisic acid (ABA) plays pivotal roles in triggering stomatal closure and facilitating adaptation of plants to drought stress. Hydrogen sulfide (H 2S), a small signaling gas molecule, is involved in ABA-dependent stomatal closure. However, how H 2S regulates ABA signaling remains largely unclear. Here, we show that ABA induces the production of H 2S catalyzed by L-CYSTEINE DESULFHYDRASE1 (DES1) in guard cells, and H 2S in turn positively regulates ABA signaling through persulfidation of Open Stomata 1 (OST1)/SNF1-RELATED PROTEIN KINASE2.6 (SnRK2.6). Two cysteine (Cys) sites, Cys131 and Cys137, which are exposed on the surface of SnRK2.6 and close to the activation loop, were identified to be persulfidated, which promotes the activity of SnRK2.6 and its interaction with ABA response element-binding factor 2 (ABF2), a transcription factor acting downstream of ABA signaling. When Cys131, Cys137, or both residues in SnRK2.6 were substituted with serine (S), H 2S-induced SnRK2.6 activity and SnRK2.6–ABF2 interaction were partially ( SnRK2. 6 C131S and SnRK2. 6 C137S) or completely ( SnRK2. 6 C131SC137S) compromised. Introduction of SnRK2. 6 C131S, SnRK2. 6 C137S, or SnRK2. 6 C131SC137S into the ost1-3 mutant could not rescue the mutant phenotype: less sensitivity to ABA- and H 2S-induced stomatal closure and Ca 2+ influx as well as increased water loss and decreased drought tolerance. Taken together, our study reveals a novel post-translational regulatory mechanism of ABA signaling whereby H 2S persulfidates SnRK2.6 to promote ABA signaling and ABA-induced stomatal closure.

服务人员 孙小满

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