Local changes in chromatin accessibility and transcriptional networks underlying the nitrate response in Arabidopsis roots

编号 040021104

推送时间 20191104

研究领域 森林培育 

年份 2019 

类型 期刊 

语种 英语

标题 Local changes in chromatin accessibility and transcriptional networks underlying the nitrate response in Arabidopsis roots

来源期刊 MOLECULAR PLANT

期 第211期

发表时间 20190914

关键词 Nitrate;  transcriptional regulation;  footprinting;  RNA polymerase II;  regulatory networks; 

摘要 Transcriptional regulation, determined by chromatin structure and regulatory elements interacting at promoter regions, is a key step in plant responses to environmental cues. Nitrate (NO3-) is a nutrient signal that regulates the expression of hundreds of genes in Arabidopsis thaliana. Here we integrate mRNA-seq, genome-wide RNA polymerase II (RNPII), ChIP-Seq and DNase-seq data sets to establish the relationship between RNPII occupancy and chromatin accessibility in response to NO3- treatments in Arabidopsis root organs. Genomic footprinting allowed us to identify in vivo regulatory elements controlling gene expression in response to NO3- treatments. NO3--modulated TF footprints are important for a rapid increase in RNPII occupancy and transcript accumulation over time. We mapped key TF regulatory interactions and functionally validated the role of NAP, a NAC-domain containing TF, as a new regulatory factor in NO3- transport. Our strategy provides a comprehensive view of transcriptional networks in response to a nutrient signal in Arabidopsis roots.

服务人员 孙小满

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