Unusual DNA-binding properties of the Arabidopsis thaliana WRKY50 transcription factor at target gene promoters

编号 040026403

推送时间 20201109

研究领域 森林培育 

年份 2020 

类型 期刊 

语种 英语

标题 Unusual DNA-binding properties of the Arabidopsis thaliana WRKY50 transcription factor at target gene promoters

来源期刊 Plant Cell Reports

期 第264期

发表时间 20201002

关键词 Electrophoretic mobility shift assay;  Parsley protoplasts;  Pep25;  Reporter gene;  Transcriptional regulation;  Transient gene expression; 

摘要 Based on the genome-wide prediction of WRKY50 target genes and the similarity of a WRKY50 binding site to WT-boxes in microbe-associated molecular pattern (MAMP)-responsive cis-regulatory modules (CRM), four WT-box containing CRMs from the promoter region of three WRKY50 target genes were investigated for their interaction with WRKY50. These target genes are DJ1E, WRKY30 and ATBBE4. Two of the four CRMs, one from DJ1E and one from WRKY30, were able to activate reporter gene expression in the presence of WRKY50. Activation requires the WT-boxes GGACTTTT, GGACTTTG from DJ1E and GGACTTTC from WRKY30. WRKY50 does not activate a second CRM from WRKY30 and the CRM from ATBBE4, both containing the WT-box TGACTTTT. In vitro gel-shift assays demonstrate WT-box-specific binding of the WRKY50 DNA-binding domain to all four CRMs. This work shows a high flexibility of WRKY50 binding site recognition beyond the classic W-box TTGACC/T.

服务人员 孙小满

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