The GIGANTEA-ENHANCED EM LEVEL complex enhances drought tolerance via regulation of abscisic acid synthesis

编号 040025304

推送时间 20200824

研究领域 森林培育 

年份 2020 

类型 期刊 

语种 英语

标题 The GIGANTEA-ENHANCED EM LEVEL complex enhances drought tolerance via regulation of abscisic acid synthesis

来源期刊 Plant Physiology

期 第253期

发表时间 20200720

关键词 Arabidopsis thaliana;  drought stress;  GIGANTEA (GI);  ENHANCED EM;  LEVEL (EEL);  9-CIS-EPOXYCAROTENOID DIOXYGENASES 3 (NCED3);  abscisic acid (ABA); 

摘要 Drought is one of the most critical environmental stresses limiting plant growth and crop productivity. The synthesis and signaling of abscisic acid (ABA), a key phytohormone in the drought stress response, is under photoperiodic control. GIGANTEA (GI), a key regulator of photoperiod-dependent flowering and the circadian rhythm, is also involved in the signaling pathways for various abiotic stresses. In this study, we isolated ENHANCED EM LEVEL (EEL)/bZIP12, a transcription factor involved in ABA signal responses, as a GI interactor in Arabidopsis thaliana. The diurnal expression of 9-CIS-EPOXYCAROTENOID DIOXYGENASE 3 (NCED3), a rate-limiting ABA biosynthetic enzyme, was reduced in the eel, gi-1, and eel gi-1 mutants under normal growth conditions. Chromatin immunoprecipitation and electrophoretic mobility shift assays revealed that EEL and GI bind directly to the ABA-Responsive Element (ABRE) motif in the NCED3 promoter. Furthermore, the eel, gi-1, and eel gi-1 mutants were hypersensitive to drought stress due to uncontrolled water loss. The transcript of NCED3, endogenous ABA levels, and stomatal closure were all reduced in the eel, gi-1, and eel gi-1 mutants under drought stress. Our results suggest that the EEL-GI complex positively regulates diurnal ABA synthesis by affecting the expression of NCED3, and contributes to the drought tolerance of Arabidopsis.

服务人员 孙小满

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